Feature Barcode Seq Level 1 Template

A template outlining metadata to use as annotations for feature barcode sequencing fastq files.

Attribute Description Required Valid Values
Component A high-level attribute for grouping attributes into templates. TRUE
Filename Name of a file. Must be included in DependsOn for all file manifest/annotation templates. TRUE
fileFormat Standard file format name or file extension TRUE csv, tsv, txt, xlsx, xls, fcs, docx, erate, rec, info, vcf, dose, tbi, fam, bim, bed, bam, h5, mtx, bai, rds, pdf, tgz, zip, fastq, parquet, geojson, tar.gz, zip, h5ad
program Name of the funding program that supported the generation of data and associated files TRUE AMP RA/SLE, AMP AIM, Community Contribution
project A sub-level attribute of `program` specifying a research initiative working to investigate particular hypotheses. TRUE RA, SLE, AIM for RA, STAMP, LOCKIT, V-CoRT, ELLIPSS
resourceType High-level classification of the file content TRUE experimental data, metadata, figure, code
nucleicAcidSource The source of the nucleic acid used as input for sequencing library fragments. Select all that apply, though in most cases only a single label is expected. TRUE poly(A) RNA, rRNA-depleted RNA, gDNA, surface protein feature barcode, intracellular protein feature barcode, antigen capture barcode, multiplexing oligo, BCR mRNA, TCR mRNA, Tn5-accessible gDNA, globin-depleted RNA, CRISPR protospacer feature barcode
targetPanel A unique or established human-readable name assigned to the panel of targets profiled in the experiment. For example, the panel of antibodies and corresponding fluorophores used in a flow cytometry experiment or panel used in a Xenium spatial transcriptomics experiment. TRUE
targetPanelSynID In most cases an accompanying metadata file should be provided that details information about the targets profiled in the experiment. This attribute links experimental data files to the target panel metadata via the synapse ID of that file. FALSE
libraryID A library label or name, unique within an experiment, used to distinguish sequencing libraries. FALSE
batch A label or identifier that is unique within a given experiment or dataset used to group data related to sample processing, sample pooling, or data collection batches. These batches often introduce technical effects that can be important to account for in downstream analyses. FALSE