biospecimenSubtype

Biospecimen status before sample is processed into a scRNA-seq library. Several scRNA-seq technologies support a variety of sample processing methods which can introduces sources of technical variation.

Valid Values Description Source
FFPE tissue formalin-fixed paraffin-embedded preservation of tissue
PFA-fixed tissue Paraformaldehyde fixation to preserve tissue, often for immunohistochemistry (IHC)
cell suspension A mixture of single or disaggregated cell suspended in a liquid solution.
flow-sorted cells A cell suspension sample that has undergone fluorescence-activated cell sorting based on protein expression and cellular composition
fresh tissue Tissue that has not been fixed or preserved
frozen tissue Tissue that has been preserved by freezing at very low temperatures
nuclei suspension Isolated cell nuclei suspended in a liquid solution