biospecimenSubtype

Biospecimen status before sample is processed into a scRNA-seq library. Several scRNA-seq technologies support a variety of sample processing methods which can introduces sources of technical variation.

Valid Values Description Source
FFPE tissue formalin-fixed paraffin-embedded preservation of tissue
PFA-fixed tissue Paraformaldehyde fixation to preserve tissue, often for immunohistochemistry (IHC)
cell or tissue lysate
cell suspension A mixture of single or disaggregated cell suspended in a liquid solution.
flow-sorted cells A cell suspension sample that has undergone fluorescence-activated cell sorting based on protein expression and cellular composition
fresh tissue Tissue that has not been fixed or preserved
frozen tissue/fluid
nuclei suspension Isolated cell nuclei suspended in a liquid solution
supernatant