FCS File Annotation Template

A template outlining metadata to be collected and applied to FCS file entities as annotations in Synapse.

Attribute Description Required Valid Values
specimenModality Label assigned to experimental data files indicating whether the data contained corresponds to a single or multiple biospecimens True multispecimen, single specimen
eventCount The total number of events detected and captured in the corresponding FCS file. In cytometry, an event corresponds to particles detected and measured by the instrument. This number corresponds to the number of rows in the FCS file and is expected to be a whole integer number. False
fileFormat Standard file format name or file extension fcs
Component A high-level attribute for grouping attributes into templates. True
assay The technology used to generate the data in this file. TRUE CyTOF, flow cytometry
dataCollectionBatch A label indicating batching that occurs during data collection, e.g., collecting data across multiple days. False
sampleProcessingBatch A label indicating batching of sample processing or preparation that occurs prior to data collection. False
individualID Unique identifier assigned to each study participant. For multi-specimen data files provide all IDs in a comma-separated list. True
biospecimenID A unique identifier assigned to specimens collected from study participants. For multi-specimen data files provide all IDs in a comma-separated list. True